Karavyraki 2022 BEC

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Karavyraki M, Gnaiger E, Porter RK (2022) Bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. https://doi.org/10.26124/bec:2022-0011

Β» Bioenerg Commun 2022.11. Open Access pdf
published online 2022-11-11Β»Watch the presentationΒ«

Karavyraki Marilena, Gnaiger Erich, Porter Richard K (2022) Bioenerg Commun

Abstract: BEC.png https://doi.org/10.26124/bec:2022-0011

In an endeavor to understand the metabolic phenotype behind oral squamous cell carcinomas, we characterized the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial abundance but lower mitochondrial oxygen consumption rates than DOK cells. The lower oxygen consumption rate in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity and lower mitochondrial complex 1 activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial abundance. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux. β€’ Keywords: oral squamous cancer cells; mitochondria; interleukin 6; dysplastic oral keratinocytes; oxygen consumption β€’ Bioblast editor: Tindle-Solomon L β€’ O2k-Network Lab: AT Innsbruck Oroboros, IE Dublin Porter RK

ORCID: Karavyraki Marilena, ORCID.png Gnaiger Erich, ORCID.png Porter Richard K

Data availability

Original files are available Open Access at Zenodo repository: https://zenodo.org/record/7325015#.Y3QcOcfP1PY


Β» Karavyraki 2022 MitoFit


Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021). We thank Rafael Moreno-Sanchez for a constructive review of our manuscript.

Labels: MiParea: Respiration  Pathology: Cancer 

Organism: Human 

Preparation: Permeabilized cells  Enzyme: Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases  Regulation: Aerobic glycolysis  Coupling state: LEAK, ROUTINE, ET  Pathway: S, ROX  HRR: Oxygraph-2k 

Crabtree effect, BEC 

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