Komlodi 2022 BEC: Difference between revisions
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Revision as of 12:52, 29 November 2022
KomlΓ³di T, Tretter L (2022) The protonmotive force β not merely membrane potential. Bioenerg Commun 2022.16. https://doi.org/10.26124/bec:2022-0016 |
Β» Bioenerg Commun 2022.16.
published online 2022-11-29
Komlodi Timea, Tretter Laszlo (BEC 2022.16) Bioenerg Commun
Abstract: https://doi.org/10.26124/bec:2022-0016
The protonmotive force pmF establishes the link between electrical and chemical components of energy transformation and coupling of oxidative phosphorylation in the mitochondrial electron transfer system. The electric part corresponds to the mitochondrial membrane potential ΞΞ¨mt and the chemical part is related to the transmembrane pH difference ΞpH. Although the contribution of ΞpH to pmF is smaller than that of ΞΞ¨mt, ΞpH plays an important role in mitochondrial transport processes and regulation of reactive oxygen species production. Measurement of both ΞΞ¨mt and ΞpH allows for calculation of pmF. Methods for monitoring ΞΞ¨mt such as fluorescence dyes are generally available, while determination of ΞpH is more challenging.
In this review, we focus on the application of the fluorescence ratiometric method using the acetoxymethyl ester form of 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF/AM) for real-time monitoring of the intramitochondrial pH in isolated mitochondria. Knowing the intra- and extramitochondrial pH allows for calculating the ΞpH. Application of specific ionophores such as nigericin or valinomycin provides the possibility to dissect the two components of the pmF. We summarize the mitochondrial processes β such as production of reactive oxygen species β for which ΞpH plays an important role.
β’ Keywords: BCECF; intramitochondrial pH; matrix pH; ΞpH; mitochondria, mitochondrial membrane potential, ΞΞ¨mt; nigercin; protonmotive force, pmF; reverse electron transfer, RET; safranin; triphenylphosphonium, TPP+; valinomycin
β’ Bioblast editor: Tindle-Solomon L
β’ O2k-Network Lab: HU Budapest Tretter L
Preprint
Labels: MiParea: Instruments;methods, mt-Membrane
Preparation: Isolated mitochondria
Enzyme: Complex V;ATP synthase
Regulation: ATP production, mt-Membrane potential, pH
Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k, O2k-Fluorometer, TPP
BEC