Difference between revisions of "MiPNet18.05 Amplex-Mouse-heart"
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/2/2a/MiPNet18.05_Amplex-Mouse-heart.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/2/2a/MiPNet18.05_Amplex-Mouse-heart.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions] | ||
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|year=2019-06-24 | |year=2019-06-24 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6. | |abstract=Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6. | ||
The [[O2k-Fluorescence LED2-Module]] is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production. | |||
:» Product: [[Oroboros O2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | :» Product: [[Oroboros O2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | ||
:» [[Permeabilized muscle fibers]] | :» [[Permeabilized muscle fibers]] | ||
|mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom | |mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom | ||
}} | }} | ||
{{Keywords H2O2}} | {{Keywords H2O2}} |
Revision as of 17:43, 13 December 2019
MiPNet18.05 Amplex-Mouse-heart
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. |
» »Versions
Oroboros (2019-06-24) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6.
The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- » Product: Oroboros O2k, O2k-Catalogue
- » Permeabilized muscle fibers
• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom