Difference between revisions of "MiPNet19.18D O2k-Series G and DatLab 6: Calibration"

Revision as of 20:16, 8 January 2018

MiPNet19.18D O2k-Series G and DatLab 6: Calibration

O2k-calibration by DatLab 6.

»

Oroboros (2015-08-08) Mitochondr Physiol Network

Abstract: Gnaiger E (2016) Oxygen calibration by DatLab 6. Mitochondr Physiol Network 19.18(D04):39-48. » Versions

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

• O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Instruments;methods

HRR: Oxygraph-2k, O2k-Protocol

O2k-Core, DatLab, DL6, DL6a7, DL7, SeriesG

DatLab 7

» DL-Protocol (DLP): O2 calibration air - This DLP provides a guide through an oxygen calibration protocol (air calibration). Version 7 beta: check for or create a folder DL-Protocol on your PC and download the DLP file to this folder. DL-Protocols are functional in DatLab 7 only.

» Template: O2 calibration List - for quality control with a track record of O₂ calibrations.

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

POS calibration

Static POS calibration = Two-point calibration of the polarographic oxygen sensor.

DatLab converts the raw signal to oxygen concentrations by applying conversion factors determined during a POS calibration comprising an air calibration and a zero calibration. For each gain a different set of calibration values is necessary. Frequently, assumed hardware problems turn out to be a simple calibration problem. This can easily be detected by observing the Raw signal.

Dynamic POS calibration = Calibration of the response time of the POS.

Related O2k-Protocols

» O2k-SOP: MiPNet06.03 POS-calibration-SOP

» MiPNet14.06 Instrumental O2 background

» DatLab

@@ Line 7: / Line 7: @@
 |journal=Mitochondr Physiol Network
 |abstract='''Gnaiger E (2016) Oxygen calibration by DatLab 6. Mitochondr Physiol Network 19.18(D04):39-48.''' » [http://www.bioblast.at/index.php/File:MiPNet19.18D_O2k-Calibration.pdf Versions]
+{{MiPNet pdf page linking to MitoPedia}}
-'''O2k-Series G Manual (DatLab 6)''' [[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the [[Oroboros O2k]] at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the [[polarographic oxygen sensor]] (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate [[oxygen solubility]] for aqueous solutions for the conversion of partial [[oxygen pressure]] into oxygen concentration ([[MiPNet06.03]]). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.
+|keywords=
-:» O2k-SOP: [[MiPNet06.03 POS-calibration-SOP]]
-:» Product: [[DatLab]], [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
-|keywords=HRR, DatLab
 |mipnetlab=AT_Innsbruck_Oroboros
 }}
@@ Line 20: / Line 16: @@
 |additional=O2k-Core, DatLab, DL6, DL6a7, DL7, SeriesG
 }}
 == DatLab 7 ==
 ::::» '''DL-Protocol''' (DLP): [[MiPNet06.03_POS-calibration-SOP#Instrumental_O2_DLP|O2 calibration air]] - This DLP provides a guide through an oxygen calibration protocol (air calibration). Version 7 beta: check for or create a folder '''DL-Protocol''' on your PC and download the DLP file to this folder. DL-Protocols are functional in DatLab 7 only.