Difference between revisions of "MiPNet19.18D O2k-Series G and DatLab 6: Calibration"
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[[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the [[Oxygraph-2k|O2k]] at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the [[polarographic oxygen sensor]] (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate [[oxygen solubility]] for aqueous solutions for the conversion of partial [[oxygen pressure]] into [[oxygen concentration]] ([[MiPNet06.03]]). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab. | [[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the [[Oxygraph-2k|O2k]] at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the [[polarographic oxygen sensor]] (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate [[oxygen solubility]] for aqueous solutions for the conversion of partial [[oxygen pressure]] into [[oxygen concentration]] ([[MiPNet06.03]]). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab. | ||
At the moment video clips are only available via the USB flash drive of the [[O2k-Compendium]]. | |||
:» Product: '''[[DatLab]] 6''', [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :» Product: '''[[DatLab]] 6''', [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
Revision as of 16:13, 19 May 2015
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OROBOROS (2015-05-15) Mitochondr Physiol Network
Abstract: Gnaiger E (2015) Oxygen calibration by DatLab. Mitochondr Physiol Network 19.18(D02):39-48.
Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the O2k at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration (MiPNet06.03). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.
At the moment video clips are only available via the USB flash drive of the O2k-Compendium.
- » Product: DatLab 6, OROBOROS O2k, O2k-Catalogue
• Keywords: HRR, DatLab
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Core, DatLab
