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MiPNet19.18D O2k-Series G and DatLab 6: Calibration

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Publications in the MiPMap
O2k-Manual
O2k-calibration by DatLab. »Bioblast pdf«

» Versions

OROBOROS (2014-10-01) Mitochondr Physiol Network

Abstract: Gnaiger E (2014) Oxygen calibration by DatLab. Mitochondr Physiol Network 19.01(D01):39-48.


Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the Oxygraph-2k O2k at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration (MiPNet06.03). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.

» Product: DatLab 6, OROBOROS O2k, O2k-Catalogue

Keywords: HRR, DatLab

O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-Core, DatLab 


Related O2k-SOP

>> MiPNet06.03 POS-Calibration-SOP
>> MiPNet14.06 InstrumentalBackground