Difference between revisions of "MiPNet21.07 MitoFit DatLab PT"

Latest revision as of 17:06, 13 December 2019

MiPNet21.07 MitoFit DatLab PT

MitoFit DatLab proficiency test.

» »Versions

Oroboros (2016-07-20) Mitochondr Physiol Network

Abstract: Plattner C, Krumschnabel G, Sumbalova Z, Doerrier C, Gnaiger E (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(02):1-3.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

» Product:DatLab

• O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Instruments;methods

Organism: Human

Preparation: Permeabilized cells

Coupling state: LEAK, OXPHOS, ET Pathway: F, N, S, Gp, CIV, Other combinations, ROX HRR: Oxygraph-2k, TIP2k, O2k-Protocol

MitoFitPublication

@@ Line 1: / Line 1: @@
+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] MitoFit DatLab proficiency test. - [[Media:MiPNet21.07 MitoFit DatLab proficiency test.pdf |»Bioblast pdf«]]
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] MitoFit DatLab proficiency test.
-|info=[http://www.bioblast.at/index.php/File:MiPNet21.07_MitoFit_DatLab_proficiency_test.pdf Versions]
+|info=[[File:PDF.jpg|100px|link=http://www.bioblast.at/images/5/5e/MiPNet21.07_MitoFit_DatLab_PT.pdf|Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet21.07_MitoFit_DatLab_PT.pdf Versions]
-|authors=OROBOROS
+|authors=Oroboros
-|year=2016-01-21
+|year=2016-07-20
 |journal=Mitochondr Physiol Network
-|abstract='''Krumschnabel G, Plattner C, Gnaiger (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(01):1-##.'''
+|abstract=Plattner C, Krumschnabel G, Sumbalova Z, Doerrier C, Gnaiger E (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(02):1-3.
-:» Instrument: [[Oxygraph-2k|O2k]], [[DatLab]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]]
+{{MiPNet pdf page linking to MitoPedia}}
-* Page in prep.
+:» Product:[[DatLab]]
+|mipnetlab=AT_Innsbruck_Oroboros
-|mipnetlab=AT_Innsbruck_OROBOROS
 }}
 {{Labeling
 |area=Instruments;methods
 |organism=Human
-|model cell lines=HEK
 |preparations=Permeabilized cells
-|couplingstates=LEAK, OXPHOS, ETS
+|couplingstates=LEAK, OXPHOS, ET
-|substratestates=CI, CII, CI&II, ROX
+|pathways=F, N, S, Gp, CIV, Other combinations, ROX
-|instruments=Oxygraph-2k, O2k-Protocol
+|instruments=Oxygraph-2k, TIP2k, O2k-Protocol
-|additional=O2k-SOP, MitoFitPublication
+|additional=MitoFitPublication
 }}
-__TOC__
-===Abstract:===
-The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background and copying of parameters for flux analysis.
-===Aims===
-# Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states.
-# Guide the user to the DatLab-Excel template for analysis.
-# Proficiency test: Two-point O<sub>2</sub> calibration, with O<sub>2</sub> solubility factor.
-# Obtain a measure of noise of instrumental O<sub>2</sub> background parameters due to variability in setting marks on 'flux'.
-# Proficiency test: Application of instrumental O<sub>2</sub> background to analysis of flux.
-===Files to download:===
-# Instructions: [[File:DatLabanalysisTest_instructions.pdf]]
-# Experimental data file: [[File:file_exp1_xx.DLD]]
-# Template: [[File:Template1 xx.xls]]
-# Calibration and Background file: [[File:file_bgr1_xx.DLD]]