MiPNet21.07 MitoFit DatLab PT

MitoFit DatLab proficiency test. - »Bioblast pdf«

» Versions

OROBOROS (2016-01-21) Mitochondr Physiol Network

Abstract: Krumschnabel G, Plattner C, Gnaiger (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(01):1-##.

» Instrument: O2k, DatLab, O2k-Catalogue

• Page in prep.

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Instruments;methods 

Organism: Human 

Preparation: Permeabilized cells 

Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, O2k-Protocol 

O2k-SOP, MitoFitPublication 

Abstract:

The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background and copying of parameters for flux analysis.

Aims

1. Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states.
2. Guide the user to the DatLab-Excel template for analysis.
3. Proficiency test: Two-point O₂ calibration, with O₂ solubility factor.
4. Obtain a measure of noise of instrumental O₂ background parameters due to variability in setting marks on 'flux'.
5. Proficiency test: Application of instrumental O₂ background to analysis of flux.

Files to download:

1. DatLab proficiency test instructions: Instructions
2. Experimental data file: file_exp1_xx.DLD
3. Template: Template1 xx.xls
4. Calibration and Background file: file_bgr1_xx.DLD