MitoKit-CII/Malonate-nv

high-resolution terminology - matching measurements at high-resolution

MitoKit-CII/Malonate-nv

Description

NV161/MitoKit-CII (diacetoxymethyl malonate) is a plasma membrane-permeable prodrug (permeable malonate; Mnanv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space.

Abbreviation: Mnanv

Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun

MitoPedia topics: Media for respirometry 

Application in HRR

Mnanv: NV161 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol^-1

Preparation of 100 mM stock solution:

1. Add 395.78 µL DMSO in 7.7 µL NV161.
2. Divide into 40 µL portions.
3. Store at -20 °C.

Preparation of 10 mM stock solution (from the 100 mM stock):

1. Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
2. Divide into 100 µL portions.
3. Store at -20 °C.

» O2k manual titrations

• Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
• Final concentration: 5 µM steps.
• Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.