MitoKit-CII/Succinate-nv: Difference between revisions
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::::# Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO. | ::::# Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO. | ||
::::# Divide into 50 ยตL portions.ย ย | ::::# Divide into 50 ยตL portions.ย ย | ||
::::# Store at -20 ยฐC. | ::::# Store 6 month at -20 ยฐC or 1 week at room temperature. | ||
:::ยป '''O2k manual titrations''' ย | :::ยป '''O2k manual titrations''' ย |
Revision as of 11:42, 20 April 2020
Description
MitoKit-CII/Succinate-nv (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space. NeuroVive #: NV118
Abbreviation: Snv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Application in HRR
- Succinate-nv: 25 mg, MW=262.2 g.mol-1
- Preparation of 1 M stock solution:
- Dissolve 25 mg Snv in 95.3 ยตL DMSO.
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO.
- Divide into 50 ยตL portions.
- Store 6 month at -20 ยฐC or 1 week at room temperature.
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- ยป O2k manual titrations
- Event name: Snv
- Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber).
- Final concentration: 50 ยตM steps.
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- ยป O2k manual titrations