Difference between revisions of "MitoOx2"
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== Application in [[ | == Application in [[HRFR]] == | ||
'''MitoOx2: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production''' | '''MitoOx2: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production''' | ||
Revision as of 14:38, 29 August 2017
- high-resolution terminology - matching measurements at high-resolution
MitoOx2
Description
Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations to measure the H2O2 production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H2O2 present) for Amplex UltraRed(R) than e.g. MiR05.
Abbreviation: MitoOx2
MitoPedia topics: Media for respirometry
Application in HRFR
MitoOx2: Mitochondrial Respiration Medium for measuring the H2O2 production
- Oxygen solubility factor of MitoOx2 at 30°C and 37°C = 0.95
Chemicals for MitoOx2
| Compound | Final conc. | FW | Company, product code and storage |
|---|---|---|---|
| KCl | 120 mM | 74.55 | Sigma 31248, store at RT |
| HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
| KH2PO4 | 10 mM | 136.09 | Sigma P-5655, store at RT |
| MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
| EGTA free acid | 0.2 mM | 380.35 | Sigma E-4378, store at RT |
| BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 °C |
adjust pH to 7.0 (room temperature) with KOH
