Myxothiazol

high-resolution terminology - matching measurements at high-resolution

Myxothiazol

Description

Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome b_L) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.

Abbreviation: Myx

Reference: MiPNet09.12

Application in HRR

Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C₂₅H₃₃N₃O₃S₂); Sigma T 5580, 1 mg, store at 4 °C: M = 487.7 g·mol^-1. Available again from Sigma with this new product number. The old product number was M 5779.

Caution: Very toxic.

Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:

1. Weigh 1 mg of myxothiazol into a small glass vial.
2. Add 2.05 mL ethanol.
3. Divide into 0.2 mL (use glass vials).
4. Store at -20 °C.

»O2k manual titrations MiPNet09.12

• Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 0.5 µM.

Preparation of 0.25mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:

1. Take 100 µL of the 1 mM stock solution.
2. Add 300 µL ethanol.

»O2k manual titrations MiPNet09.12

• Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 0.5 µM.

Compare

• Antimycin A is less expensive than Myx but induces higher ROS production.

MitoPedia topics: Inhibitor