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Difference between revisions of "NextGen-O2k Documentation"

From Bioblast
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* '''Q-service'''
* '''Q-service'''


[https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be  Assembly of the reference electrode]
[https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be  Assembly of the reference electrode]<br>
[https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
[https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]<br>
[https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
[https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]<br>


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* ''' Cleaning Q-Sensors'''
* ''' Cleaning Q-Sensors'''
[https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]
[https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]<br>
 
[https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]
[https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]


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<div class="mw-collapsible-content">


[https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]<br>
[https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the smart-fluo sensor]<br


[https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]
>[https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
 
[https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the smart-fluo sensor]
 
[https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
 
</div>
</div>
</div>
</div>
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<div class="mw-collapsible-content">
<div class="mw-collapsible-content">


[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]<br>
 
[https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]<br>
[https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]
[https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
[https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]



Revision as of 09:13, 26 May 2021

Template:MitoFit highlights page name

NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP3: Documentation



Abstract

In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.

Achievements

Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.
Project Year 2: Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module.

Progress and next steps

See » Track record

PlayVideo.jpg NextGen-O2k Videosupport clips

Click to expand or collaps
Click to expand or collaps
Click to expand or collaps
Click to expand or collaps


O2k-Manual.jpg NextGen-O2k Manuals

» DatLab8.0: CV-Module
» NextGen-O2k: Q-Module
» Oxygen flux_analysis - DatLab 7.4
» Safranin Analysis Template
» General template for mt-membrane potential analysis
» H2O2 flux analysis