O2k-Open Support
A good O2k-Troubleshooting procedure helps to localize and resolve problems encountered with the OROBOROS Oxygraph-2k as fast and as cost effective as possible. Most problems can be located easily on a particular component that can be serviced or - if necessary - replaced by the user, avoiding to ship the entire O2k. Some specific potential problems, e.g. a non-functioning Peltier unit, can be detected directly by a single observation followed by specific instructions for this case. However, usually it is best to follow a general procedure regardless of the initially observed problem. This is especially true for problems that may have different causes or are vague in their description. "Unstable signal", "no signal", "slow response" all fall within this category. The general O2k-Troubleshooting process described below will help to locate most hardware problems.
General O2k-Troubleshooting process
To find hardware problems all uncertainties implied by a biological experiment have to be eliminated. A Datlab file obtained with actual biological material is not suitable for troubleshooting purposes.
- Perform a POS Sensor Test and analyze its results.
- Locate the problem utilizing the two-chamber approach of the O2k by strictly following the procedure described on the Switching components page. This also involves several more POS sensor tests.
Specific problems that do not require a sensor test
DatLab error messages
- DatLab Error Message: Block temperature: ERROR: Average block temperature over past 10 min has not reached set temperature.
- DatLab Error Message: Line has wrong length: ERROR: Line has wrong length: This message appears when a signal line has not been transmitted properly from the Oxygraph-2k to DatLab.
Other problems that do not require a POS sensor test
- Problems related only to the pX channel or the additional amperometric channel.
- Jumping or blocked stirring bar.
- High oxygen flux at closed chamber.
How to find O2k-troubleshooting and technical service information in Bioblast
- You can use the standard Wiki search function in the right upper corner of most pages.
- Use the Browse data function of Semantic Wiki. Follow the link to Service, then refine your search further by selecting appropriate keywords from the Technical service filter (multiple selections possible - click arrow to add another value). You will get a refined list of pages that fit your search criteria.
The link above is equivalent to clicking Browse data from the sidebar and then selecting first the category OroboroPedia (from the Box on the right side) and then the subcategory Service.
- All technical service and troubleshooting pages are listed below.
Scientific Service
All Scientific Service pages
All Technical Service and O2k-Troubleshooting Pages
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