Difference between revisions of "OctPGM pathway control state"
From Bioblast
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::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] | ::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] | ||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | ::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | ||
::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 pce D007]] | ::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 ce-pce D007]] | ||
:::*[[SUIT-015]] | :::*[[SUIT-015]] | ||
Revision as of 16:13, 27 March 2019
- high-resolution terminology - matching measurements at high-resolution
OctPGM pathway control state
Description
OctPGM: Octanoylcarnitine & Pyruvate & Glutamate & Malate.
MitoPathway control state: FN
SUIT protocols: SUIT-002
- This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<1 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare malate anaplerotic pathway control state, M alone), and pyruvate and glutamate is added to compare FAO as the background state with FN as the reference state.
Abbreviation: OctPGM
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia concepts:
SUIT state
OctPGML
FN pathway in the LEAK state can be evaluated in the following SUIT protocols:
OctPGMP
FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007
-
OctPGME
FN pathway in the ET state can be evaluated in the following SUIT protocols:
