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Oxygen sensor test |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- The O2 sensor test is described as an OroboPOS standard operating procedure: Polarographic oxygen sensors: calibration, accuracy and quality control SOP. »MiPNet06.03 POS-Calibration-SOP«
When should an O2-sensor test be performed?
- After switching on the O2k, every day: air calibration and stirrer test.
- Zero oxygen calibration: from time to time over weeks; bracketing zero oxygen calibrations when working at low oxygen.
- After application of a new membrane and O2-sensor service: In some cases, the signal of the OroboPOS improves (higher signal stability, less noise, shorter response time), when the O2k remains switched on over night (O2k-Chambers filled with 70% EtOH).
- When there is any doubt about instrumental performance.
- During troubleshooting when switching components between the two chambers, a quick sensor test is performed after each step (stirrer test, sensor signal).
Evaluation of an O2 sensor test
Most parameters are best viewed by observing the Raw Signal. It will be helpful to compare the obtained test with the test of a functioning system, e.g. from the DatLab demo file.
Informations gained by the sensor test include:
Signal stability at air saturation
Flux at closed chamber near to air saturation
Raw signal at zero oxygen (zero current)
Signal stability at zero oxygen
Consequences of a Sensor test
Hardware problems are detected by assessing the parameters listed above in 'Evaluation of a Sensor test'.
If a hardware problem was observed in a 'stand alone' sensor test, please follow the link for the problematic parameter. Frequently it will be necessary to find the faulty component by locating a problem.
If a sensor test was already performed as part of locating a problem the information in which chamber of the oxygraph the problem appeared will be used in the further process of locating a problem.
