Difference between revisions of "Palmitate"
From Bioblast
| (10 intermediate revisions by 5 users not shown) | |||
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=Paa | |abbr=Paa | ||
|description='''Palmitate''' is a term for the salts and esters of palmitic acid (CH<sub>3</sub>(CH<sub>2</sub>)<sub>14</sub>COOH). Palmitic acid is the first fatty acid produced during fatty acid synthesis and the precursor to longer fatty acids. Palmitate negatively feeds back on acetyl-CoA carboxylase (ACC), which is responsible for converting acetyl-CoA to malonyl-CoA, which in turn is used to add to the growing acyl chain, thus preventing further palmitate generation. | |description='''Palmitate''' is a term for the salts and esters of palmitic acid (CH<sub>3</sub>(CH<sub>2</sub>)<sub>14</sub>COOH). Palmitic acid is the first fatty acid produced during fatty acid synthesis and the precursor to longer fatty acids. Palmitate negatively feeds back on acetyl-CoA carboxylase (ACC), which is responsible for converting acetyl-CoA to malonyl-CoA, which in turn is used to add to the growing acyl chain, thus preventing further palmitate generation. In order to dissolve the water-insoluble sodium palmitate, [[Bovine serum albumine| BSA]] is needed to form the water-soluble compound called palmitate:BSA. | ||
|type=Substrate ETS | |type=Substrate ETS | ||
}} | |||
__TOC__ | |||
== Application in [[HRR]] == | |||
::: '''Paa: Sodium palmitate''' (Sigma P 9767, 5 g, store at 4 °C; M = 278.41 g·mol<sup>-1</sup>) | |||
:::: '''Preparation of 150 mM NaCl stock solution.''' | |||
::::# Weigh 438.3 mg NaCl directly into a glass beaker. | |||
::::# Add 50 mL H<sub>2</sub>O and mix it together. | |||
:::: '''Preparation of 1.66 mM BSA stock solution.''' | |||
::::# Weigh 5 g BSA. (66kDa=66000Da=66000g/mol) | |||
::::# Add 45.6 mL 150 mM NaCl solution and mix it. | |||
:::: '''Preparation of 10 mM sodium palmitate solution.''' | |||
::::# Weigh 27.84 mg of sodium palmitate directly into a 10 mL glass beaker. | |||
::::# Add 10 mL H<sub>2</sub>O. | |||
::::# Add 10 mL 1.66 mM BSA stock solution. | |||
::::# Mix it for 1 hour at 70 °C. | |||
::::# Store at -20 °C in Eppendorf tubes. | |||
{{MitoPedia concepts|type=Substrate ETS | |||
}} | }} | ||
{{MitoPedia methods|type=Substrate ETS | {{MitoPedia methods|type=Substrate ETS | ||
}} | |||
{{MitoPedia O2k and high-resolution respirometry|type=Substrate ETS | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
| Line 11: | Line 32: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |additional=MitoPedia:FAT4BRAIN | ||
}} | }} | ||
Latest revision as of 17:08, 18 January 2022
- high-resolution terminology - matching measurements at high-resolution
Palmitate
Description
Palmitate is a term for the salts and esters of palmitic acid (CH3(CH2)14COOH). Palmitic acid is the first fatty acid produced during fatty acid synthesis and the precursor to longer fatty acids. Palmitate negatively feeds back on acetyl-CoA carboxylase (ACC), which is responsible for converting acetyl-CoA to malonyl-CoA, which in turn is used to add to the growing acyl chain, thus preventing further palmitate generation. In order to dissolve the water-insoluble sodium palmitate, BSA is needed to form the water-soluble compound called palmitate:BSA.
Abbreviation: Paa
Application in HRR
- Paa: Sodium palmitate (Sigma P 9767, 5 g, store at 4 °C; M = 278.41 g·mol-1)
- Preparation of 150 mM NaCl stock solution.
- Weigh 438.3 mg NaCl directly into a glass beaker.
- Add 50 mL H2O and mix it together.
- Preparation of 1.66 mM BSA stock solution.
- Weigh 5 g BSA. (66kDa=66000Da=66000g/mol)
- Add 45.6 mL 150 mM NaCl solution and mix it.
- Preparation of 10 mM sodium palmitate solution.
- Weigh 27.84 mg of sodium palmitate directly into a 10 mL glass beaker.
- Add 10 mL H2O.
- Add 10 mL 1.66 mM BSA stock solution.
- Mix it for 1 hour at 70 °C.
- Store at -20 °C in Eppendorf tubes.
- Preparation of 150 mM NaCl stock solution.
MitoPedia topics: Substrate and metabolite
Labels:
MitoPedia:FAT4BRAIN
