Difference between revisions of "Picard 2011 PLoS One"
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{{Publication | {{Publication | ||
|title=Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C, Hepple RT (2011) Mitochondrial structure and function are disrupted by standard isolation methods. PLoS One 6:e18317. | |title=Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C, Hepple RT (2011) Mitochondrial structure and function are disrupted by standard isolation methods. PLoS One 6:e18317. | ||
|info=[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065478/?tool=pubmed PMID:21512578 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065478/?tool=pubmed PMID: 21512578 Open Access] | ||
|authors=Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C, Hepple RT | |authors=Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C, Hepple RT | ||
|year=2011 | |year=2011 | ||
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mitochondria and [[Permeabilized muscle fibre|permeabilized myofibers]]. Whereas mitochondrial isolation removes a portion of the mitochondria from | mitochondria and [[Permeabilized muscle fibre|permeabilized myofibers]]. Whereas mitochondrial isolation removes a portion of the mitochondria from | ||
their cellular environment, myofiber permeabilization preserves mitochondrial morphology and functional interactions with | their cellular environment, myofiber permeabilization preserves mitochondrial morphology and functional interactions with | ||
other intracellular components. Despite this, isolated mitochondria remain the most commonly used method to infer in vivo | other intracellular components. Despite this, isolated mitochondria remain the most commonly used method to infer ''in vivo'' | ||
mitochondrial function. In this study, we directly compared measures of several key aspects of mitochondrial function in | mitochondrial function. In this study, we directly compared measures of several key aspects of mitochondrial function in | ||
both isolated mitochondria and permeabilized myofibers of rat gastrocnemius muscle. Here we show that mitochondrial | both isolated mitochondria and permeabilized myofibers of rat gastrocnemius muscle. Here we show that mitochondrial | ||
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to a Ca2+ challenge; iii) differentially altered mitochondrial respiration depending upon the respiratory conditions; and iv) | to a Ca2+ challenge; iii) differentially altered mitochondrial respiration depending upon the respiratory conditions; and iv) | ||
dramatically increased [[Measuring hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> production]]. These alterations are qualitatively similar to the changes in mitochondrial structure | dramatically increased [[Measuring hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> production]]. These alterations are qualitatively similar to the changes in mitochondrial structure | ||
and function observed in vivo after cellular stress-induced mitochondrial fragmentation, but are generally of much greater | and function observed ''in vivo'' after cellular stress-induced mitochondrial fragmentation, but are generally of much greater | ||
magnitude. Furthermore, mitochondrial isolation markedly altered electron transport chain protein stoichiometry. | magnitude. Furthermore, mitochondrial isolation markedly altered electron transport chain protein stoichiometry. | ||
Collectively, our results demonstrate that isolated mitochondria possess functional characteristics that differ fundamentally | Collectively, our results demonstrate that isolated mitochondria possess functional characteristics that differ fundamentally | ||
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studying mitochondrial function in tissue preparations where mitochondrial structure is preserved and all mitochondria are | studying mitochondrial function in tissue preparations where mitochondrial structure is preserved and all mitochondria are | ||
represented. | represented. | ||
|keywords=Isolated mitochondria, | |keywords=Isolated mitochondria, Permeabilized myofibers, ROS | ||
|mipnetlab=CA Montreal Hepple RT, | |mipnetlab=CA Montreal Hepple RT, FR Villeurbanne Romestaing C | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
Revision as of 15:23, 19 March 2015
| Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C, Hepple RT (2011) Mitochondrial structure and function are disrupted by standard isolation methods. PLoS One 6:e18317. |
Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C, Hepple RT (2011) PLoS One
Abstract: Mitochondria regulate critical components of cellular function via ATP production, reactive oxygen species production, Ca2+ handling and apoptotic signaling. Two classical methods exist to study mitochondrial function of skeletal muscles: isolated mitochondria and permeabilized myofibers. Whereas mitochondrial isolation removes a portion of the mitochondria from their cellular environment, myofiber permeabilization preserves mitochondrial morphology and functional interactions with other intracellular components. Despite this, isolated mitochondria remain the most commonly used method to infer in vivo mitochondrial function. In this study, we directly compared measures of several key aspects of mitochondrial function in both isolated mitochondria and permeabilized myofibers of rat gastrocnemius muscle. Here we show that mitochondrial isolation i) induced fragmented organelle morphology; ii) dramatically sensitized the permeability transition pore sensitivity to a Ca2+ challenge; iii) differentially altered mitochondrial respiration depending upon the respiratory conditions; and iv) dramatically increased H2O2 production. These alterations are qualitatively similar to the changes in mitochondrial structure and function observed in vivo after cellular stress-induced mitochondrial fragmentation, but are generally of much greater magnitude. Furthermore, mitochondrial isolation markedly altered electron transport chain protein stoichiometry. Collectively, our results demonstrate that isolated mitochondria possess functional characteristics that differ fundamentally from those of intact mitochondria in permeabilized myofibers. Our work and that of others underscores the importance of studying mitochondrial function in tissue preparations where mitochondrial structure is preserved and all mitochondria are represented. • Keywords: Isolated mitochondria, Permeabilized myofibers, ROS
• O2k-Network Lab: CA Montreal Hepple RT, FR Villeurbanne Romestaing C
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue, Isolated mitochondria
HRR: Oxygraph-2k
- See discussion: Talk:Permeabilized muscle fibres
