Radogna 2021 Methods Mol Biol
Radogna F, Gerard D, Dicato M, Diederich M (2021) Assessment of mitochondrial cell metabolism by respiratory chain electron flow assays. Methods Mol Biol 2276:129-141. doi: 10.1007/978-1-0716-1266-8_9 |
Radogna F, GΓ©rard D, Dicato M, Diederich M (2021) Methods Mol Biol
Abstract: As the powerhouse of the cell, mitochondria, plays a crucial role in many aspects of life, whereby mitochondrial dysfunctions are associated with pathogenesis of many diseases, like neurodegenerative diseases, obesity, cancer, and metabolic as well as cardiovascular disorders. Mitochondria analysis frequently starts with isolation and enrichment procedures, which have become increasingly important in biomedical research. Unfortunately, isolation procedures can easily cause changes in the structural integrity of mitochondria during in vitro handling having impact on their function. This carries the risk that conclusions about isolated mitochondria may be drawn on the basis of experimental artifacts. Here we critically review a commonly used isolation procedure for mitochondria utilizing differential (gradient) centrifugation and depict major challenges to achieve "functional" mitochondria as basis for comprehensive physiological studies.
β’ Bioblast editor: Gnaiger E
Labels:
Enzyme: Complex II;succinate dehydrogenase
Correction: FADH2 and Complex II
- FADH2 is shown as the substrate feeding electrons into Complex II (CII). This is wrong and requires correction - for details see Gnaiger (2024).
- Gnaiger E (2024) Complex II ambiguities β FADH2 in the electron transfer system. J Biol Chem 300:105470. https://doi.org/10.1016/j.jbc.2023.105470 - Β»Bioblast linkΒ«