Difference between revisions of "Rotenone"
| Line 13: | Line 13: | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Rot: Rotenone''' ( | '''Rot: Rotenone''' (C<sub>23</sub>H<sub>22</sub>O<sub>6</sub>); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%). | ||
<span style="color:#8B008B"> '''Caution:''' Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!</span> | <span style="color:#8B008B"> '''Caution:''' Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!</span>. | ||
| Line 25: | Line 25: | ||
'''Oxygraph-2k manual titrations | '''Oxygraph-2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 1 µl using a 10 µl syringe. | ::* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber). | ||
::* Final | ::* Final concentration: 0.5 µM. | ||
== Inhibition of CI by rotenone == | == Inhibition of CI by rotenone == | ||
* | * Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384–392. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724837/ >>PMC2724837] | ||
Revision as of 15:46, 2 May 2014
- high-resolution terminology - matching measurements at high-resolution
Rotenone
Description
Rotenone is an (synonym. 2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on) inhibitor of complex I via inhibition of NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12, MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Rot: Rotenone (C23H22O6); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%).
Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!.
Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):
- 1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).
- 2. Divide into 100 µl aliquots (use dark glass vials).
- 3) Store frozen at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 µM.
Inhibition of CI by rotenone
- Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384–392. >>PMC2724837
