Difference between revisions of "SUIT-003 O2 ce D028"
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{{MitoPedia | {{MitoPedia | ||
|description=[[File:ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama.png|200px]] | |||
|description=[[File: | |||
|info='''A''' CCP(S) | |info='''A''' CCP(S) | ||
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|mitopedia concept=SUIT protocol, SUIT B | |mitopedia concept=SUIT protocol, SUIT B | ||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' linear cell [[coupling control protocol]] (ceCCP) | ::: '''[[SUIT protocol pattern]]:''' linear cell [[coupling control protocol]] (ceCCP) | ||
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[[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | [[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | ||
:::* ''' | :::* '''ce4Rot,ce5S;ce6Ama''' | ||
:::: ''' | :::: '''ce4Rot''': In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of antimycin A. | ||
:::: ''' | :::: '''ce5S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated. | ||
:::: ''' | :::: '''ce6Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 14:49, 29 November 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
Reference: A CCP(S)
MitoPedia concepts:
SUIT protocol,
SUIT B
- SUIT protocol pattern: linear cell coupling control protocol (ceCCP)
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- ce4Rot,ce5S;ce6Ama
- ce4Rot: In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- ce5S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- ce6Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
