Difference between revisions of "SUIT-003 O2 ce D028"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=ceS | |||
|description=[[File:ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama.png|200px]] | |description=[[File:ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama.png|200px]] | ||
|info='''A''' CCP(S) | |info='''A''' CCP(S) | ||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' linear cell [[coupling control protocol]] ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama | |||
::: '''[[SUIT protocol pattern]]:''' linear cell [[coupling control protocol]] | |||
Β | |||
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:::: '''ce5S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated. | :::: '''ce5S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated. | ||
:::: '''ce6Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''ce6Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT A | |||
}} | |||
{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
}} |
Revision as of 09:22, 15 January 2019
Description
Abbreviation: ceS
Reference: A CCP(S)
- SUIT protocol pattern: linear cell coupling control protocol ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- ce4Rot,ce5S;ce6Ama
- ce4Rot: In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- ce5S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- ce6Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts: SUIT A
MitoPedia methods:
Respirometry