Difference between revisions of "Safranin"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Saf | |abbr=Saf | ||
|description='''Safranin''' is one of the most established dyes for measuring mitochondrial membrane potential by [[fluorometry]]. It is an [[extrinsic fluorophores |extrinsic fluorophore]] with an excitation wavelength of 495 nm and emission wavelength of 587 nm. | |description='''Safranin''' is one of the most established dyes for measuring mitochondrial membrane potential by [[fluorometry]]. It is an [[extrinsic fluorophores |extrinsic fluorophore]] with an excitation wavelength of 495 nm and emission wavelength of 587 nm. Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system. | ||
|info=[[Kauppinen 1984 Am J Physiol]], [[Krumschnabel 2014 Methods Enzymol]] | |info=[[Kauppinen 1984 Am J Physiol]], [[Krumschnabel 2014 Methods Enzymol]], [[MiPNet20.13 Safranin mt-membranepotential]] | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry | ||
}} | }} | ||
{{MitoPedia SUIT}} | |||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
== Synonyms == | == Synonyms == | ||
Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2 | Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2 | ||
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== Application in high-resolution respirometry == | == Application in high-resolution respirometry == | ||
At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.<ref> Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Bioblast link«]]</ref> | |||
[[Image:O2k-Protocols.jpg|40px|link=O2k-Protocols|O2k-Protocols]] '''O2k-Protocol''': O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. [[MiPNet20.13 Safranin mt-membranepotential| »MiPNet20.13«]] | [[Image:O2k-Protocols.jpg|40px|link=O2k-Protocols|O2k-Protocols]] '''O2k-Protocol''': O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. [[MiPNet20.13 Safranin mt-membranepotential| »MiPNet20.13«]] | ||
Revision as of 22:02, 6 February 2016
- high-resolution terminology - matching measurements at high-resolution
Safranin
Description
Safranin is one of the most established dyes for measuring mitochondrial membrane potential by fluorometry. It is an extrinsic fluorophore with an excitation wavelength of 495 nm and emission wavelength of 587 nm. Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system.
Abbreviation: Saf
Reference: Kauppinen 1984 Am J Physiol, Krumschnabel 2014 Methods Enzymol, MiPNet20.13 Safranin mt-membranepotential
MitoPedia methods: Fluorometry
Synonyms
Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2
Signal and output
- Signal: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [Amp]) as the primary signal.
- Output: The focus of the output with Safranin is on Type C: Force, mt-membrane potential.
Application in high-resolution respirometry
At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.[1]
O2k-Protocol: O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. »MiPNet20.13«
References
- ↑ Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Bioblast link«
