Difference between revisions of "Safranin"
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== Signal and output == | == Signal and output == | ||
:::# Signal: The [[O2k-Fluo LED2-Module]] is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [ | :::# Signal: The [[O2k-Fluo LED2-Module]] is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [A]) as the primary signal. | ||
:::# Output: The focus of the output with Safranin is on '''[[O2k signals and output| Type C]]''': Force, mt-membrane potential. | :::# Output: The focus of the output with Safranin is on '''[[O2k signals and output| Type C]]''': Force, mt-membrane potential. | ||
Revision as of 03:55, 7 August 2017
- high-resolution terminology - matching measurements at high-resolution
Safranin
Description
Safranin is one of the most established dyes for measuring mitochondrial membrane potential by fluorometry. It is an extrinsic fluorophore with an excitation wavelength of 495 nm and emission wavelength of 587 nm. Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system.
Abbreviation: Saf
Reference: Kauppinen 1984 Am J Physiol, Krumschnabel 2014 Methods Enzymol, MiPNet20.13 Safranin mt-membranepotential
MitoPedia methods:
Fluorometry
Synonyms
- Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2
Signal and output
- Signal: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [A]) as the primary signal.
- Output: The focus of the output with Safranin is on Type C: Force, mt-membrane potential.
Application in high-resolution respirometry
- At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.[1]
O2k-Protocol: O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. »MiPNet20.13«
