Difference between revisions of "Salicylhydroxamic acid"

Revision as of 10:35, 11 September 2020

high-resolution terminology - matching measurements at high-resolution

Salicylhydroxamic acid

Description

Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.

Abbreviation: SHAM

Reference: Parrino_2000_J_Exp_Biol

Application in HRR

SHAM: Salicylhydroxamic acid (99%; HOC₆H₄CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14.

Caution: Irritant!

Preparation of 20 mM stock solution (dissolved in DMSO):

Weigh 6.1 mg of SHAM.
Dissolve in 2 mL of DMSO.
Divide into 0.2 mL portions
Store at -20 °C.

» O2k Manual Titrations: MiPNet09.12 O2k-Titrations

Titration volume: 100 µL
Final conc. in 2 mL O2k chamber: 1 mM

MitoPedia topics: Inhibitor

@@ Line 4: / Line 4: @@
 |info=[[Parrino_2000_J_Exp_Biol]]
 }}
+__TOC__
 == Application in [[HRR]] ==
 ::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14.
+::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span>
-<span style="color:#8B008B"> '''Caution:''' Irritant!</span>
+::::'''Preparation of 20 mM stock solution (dissolved in DMSO)''':
-:::'''Preparation of 20 mM stock solution (dissolved in DMSO)''':
 ::::# Weigh 6.1 mg of SHAM.
 ::::# Dissolve in 2 mL of DMSO.
@@ Line 18: / Line 17: @@
 ::::# Store at -20 °C.
-::: '''O2k Manual Titrations:'''  [[MiPNet09.12 O2k-Titrations]]
+:::» '''O2k Manual Titrations:'''  [[MiPNet09.12 O2k-Titrations]]
 ::::* Titration volume: 100 µL