Difference between revisions of "Salicylhydroxamic acid"
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'''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14. | '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14. | ||
<span style="color:#8B008B"> '''Caution:''' Irritant!</span> | |||
:::'''Preparation of 20 mM stock solution (dissolved in DMSO)''': | |||
::::# Weigh 6.1 mg of SHAM. | |||
::::# Dissolve in 2 ml of DMSO. | |||
::::# Divide into 0.2 mL portions | |||
::::# Store at -20 °C. | |||
::: '''O2k Manual Titrations:''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: | |||
::::* Final conc. in 2 mL O2k-chamber: | |||
Revision as of 15:26, 9 April 2019
- high-resolution terminology - matching measurements at high-resolution
Salicylhydroxamic acid
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the cytochrome pathway and through cytochrome IV, allowing observation of the operation of the cytochrome pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
MitoPedia topics:
Inhibitor
Application in HRR
SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14.
Caution: Irritant!
- Preparation of 20 mM stock solution (dissolved in DMSO):
- Weigh 6.1 mg of SHAM.
- Dissolve in 2 ml of DMSO.
- Divide into 0.2 mL portions
- Store at -20 °C.
- Preparation of 20 mM stock solution (dissolved in DMSO):
- O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume:
- Final conc. in 2 mL O2k-chamber:
