Difference between revisions of "Saponin"
From Bioblast
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|description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations.Β Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | |description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations.Β Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | ||
|info=[[Pesta_2012_Methods_Mol_Biol|Pesta, Gnaiger 2012]] | |info=[[Pesta_2012_Methods_Mol_Biol|Pesta, Gnaiger 2012]] | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Permeabilization agent | |mitopedia topic=Permeabilization agent | ||
}} | }} | ||
== Application in [[ | == Application in [[HRR]] == | ||
'''Sap: Saponin''' (from quillaja bark); Sigma S 7900, 25 g, store at RT. | '''Sap: Saponin''' (from quillaja bark); Sigma S 7900, 25 g, store at RT. | ||
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'''Preparation of 5 mg/ | '''Preparation of 5 mg/mL stock solution (dissolved in''' [[Biopsy preservation solution|BIOPS]]): | ||
::1) Weigh 5 mg of saponin. | ::1) Weigh 5 mg of saponin. | ||
::2) Dissolve in 1 | ::2) Dissolve in 1 mL BIOPS. | ||
β Use 50 Β΅g saponin/ | β Use 50 Β΅g saponin/mL [[Biopsy preservation solution|BIOPS]]: add 20 Β΅L of 5 mg/mL saponin stock solution per 2 mL BIOPS. |
Revision as of 13:21, 17 March 2019
Description
Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
Reference: Pesta, Gnaiger 2012
MitoPedia topics:
Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S 7900, 25 g, store at RT.
Comment: Saponin from Quillaja Bark has the product numbers S 7900 and S 2149, but S 2149 is withdrawn from Sigma.
Caution: Prepare fresh everyday!
Preparation of 5 mg/mL stock solution (dissolved in BIOPS):
- 1) Weigh 5 mg of saponin.
- 2) Dissolve in 1 mL BIOPS.
β Use 50 Β΅g saponin/mL BIOPS: add 20 Β΅L of 5 mg/mL saponin stock solution per 2 mL BIOPS.