Difference between revisions of "Saponin"
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== Application in [[ | == Application in [[HRFR]] == | ||
'''Sap: Saponin''' (from quillaja bark); Sigma S 7900, 25 g, store at RT. | '''Sap: Saponin''' (from quillaja bark); Sigma S 7900, 25 g, store at RT. | ||
Revision as of 14:36, 29 August 2017
- high-resolution terminology - matching measurements at high-resolution
Saponin
Description
Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
Reference: Pesta, Gnaiger 2012
MitoPedia topics: Permeabilization agent
Application in HRFR
Sap: Saponin (from quillaja bark); Sigma S 7900, 25 g, store at RT.
Comment: Saponin from Quillaja Bark has the product numbers S 7900 and S 2149, but S 2149 is withdrawn from Sigma.
Caution: Prepare fresh everyday!
Preparation of 5 mg/ml stock solution (dissolved in BIOPS):
- 1) Weigh 5 mg of saponin.
- 2) Dissolve in 1 ml BIOPS.
→ Use 50 µg saponin/ml BIOPS: add 20 µl of 5 mg/ml saponin stock solution per 2 ml BIOPS.
