Difference between revisions of "Sodium fluoride"
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|description='''Sodium fluoride (NaF)''' is used in combination with [[beryllium sulfate]] to form beryllium trifluoride (BeF<sup>3−</sup>), to inhibit the [[ATP synthase]] if it is exposed by disruption of the mitochondrial membranes. | |description='''Sodium fluoride (NaF)''' is used in combination with [[beryllium sulfate]] to form beryllium trifluoride (BeF<sup>3−</sup>), to inhibit the [[ATP synthase]] if it is exposed by disruption of the mitochondrial membranes. | ||
}} | }} | ||
== Application in [[HRR]] == | |||
__TOC__ | __TOC__ | ||
{{Chemical_description | {{Chemical_description | ||
|abbr=NaF | |abbr=NaF | ||
|trivial name= | |trivial name= | ||
|complete name=sodium | |complete name=sodium fluoride | ||
|chem formula=NaF | |chem formula=NaF | ||
|molar mass= | |molar mass=41.99 | ||
|vendor= | |vendor= Sigma-Aldrich | ||
|product number= | |product number= S7920 | ||
|store at= | |store at=RT | ||
|sensitivity= | |sensitivity= | ||
|cas= | |cas= | ||
| Line 24: | Line 22: | ||
:::: '''Preparation of 500 mM stock solution''' (dissolved in H<sub>2</sub>O) | :::: '''Preparation of 500 mM stock solution''' (dissolved in H<sub>2</sub>O) | ||
::::# Weigh 209.95 mg of NaF. | |||
::::# Dissolve in 10 mL distilled H2O under the fume hood. | |||
::::# Divide in aliquots. | |||
::::# Store at +4 °C. | |||
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 20 µL using a 25 µL syringe (2 mL O2k-chamber). | |||
::::* Final concentration: 5 mM. | |||
== References == | == References == | ||
::::* [ | ::::* [[Chinopoulos 2014 Methods Enzymol]] | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
Latest revision as of 12:59, 26 April 2024
- high-resolution terminology - matching measurements at high-resolution
Sodium fluoride
Description
Sodium fluoride (NaF) is used in combination with beryllium sulfate to form beryllium trifluoride (BeF3−), to inhibit the ATP synthase if it is exposed by disruption of the mitochondrial membranes.
Abbreviation: NaF
Application in HRR
- NaF: (sodium fluoride; NaF), Sigma-Aldrich: S7920, store at RT, M = 41.99 g·mol-1
- Preparation of 500 mM stock solution (dissolved in H2O)
- Weigh 209.95 mg of NaF.
- Dissolve in 10 mL distilled H2O under the fume hood.
- Divide in aliquots.
- Store at +4 °C.
- Preparation of 500 mM stock solution (dissolved in H2O)
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 20 µL using a 25 µL syringe (2 mL O2k-chamber).
- Final concentration: 5 mM.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
References
MitoPedia topics: Inhibitor
