Difference between revisions of "Subsample"
From Bioblast
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|description='''Subsamples''' can be obtained (1) from a homogenous [[sample]] (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. Subsamples may be used for (i) application of different types of [[assay]] (e.g. for measurement of respiration and enzyme activities), and (ii) a number of [[repetitions]], ''n'', of the same assay on the same sample. | |description='''Subsamples''' can be obtained (1) from a homogenous [[sample]] (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. Subsamples may be used for (i) application of different types of [[assay]] (e.g. for measurement of respiration and enzyme activities), and (ii) a number of [[repetitions]], ''n'', of the same assay on the same sample. | ||
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{{MitoPedia concepts | {{MitoPedia concepts}} | ||
|mitopedia | {{MitoPedia methods}} | ||
{{MitoPedia O2k and high-resolution respirometry | |||
|mitopedia O2k and high-resolution respirometry=Oroboros QM | |||
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{{MitoPedia topics}} | {{MitoPedia topics}} | ||
Contributed by [[Gnaiger E]] 2016-02-11; last update 2016-05-28. | Contributed by [[Gnaiger E]] 2016-02-11; last update 2016-05-28. | ||
{{Template:MitoFit-QCS}} | {{Template:MitoFit-QCS}} | ||
Revision as of 05:52, 19 July 2022
- high-resolution terminology - matching measurements at high-resolution
Subsample
Description
Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. Subsamples may be used for (i) application of different types of assay (e.g. for measurement of respiration and enzyme activities), and (ii) a number of repetitions, n, of the same assay on the same sample.
Abbreviation: n
MitoPedia O2k and high-resolution respirometry:
Oroboros QM
Contributed by Gnaiger E 2016-02-11; last update 2016-05-28.
