Difference between revisions of "Substrate-uncoupler-inhibitor titration"
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|type=Respiration | |type=Respiration | ||
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[[File: | [[File:Pesta_2012_Methods_Mol_Biol_Fig7A.jpg|right|500px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]] | ||
==SUIT protocols== | ==SUIT protocols== | ||
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==References== | ==References== | ||
* [[Boushel 2007 Diabetologia]] | |||
* [[ | * [[Gnaiger 2007 MitoPathways]] >> ''New edition:'' [[Gnaiger 2014 MitoPathways]] | ||
* [[ | |||
* [[Gnaiger 2009 Int J Biochem Cell Biol]] | * [[Gnaiger 2009 Int J Biochem Cell Biol]] | ||
* [[ | * [[Lemieux 2011 Int J Biochem Cell Biol]] | ||
* [[ | * [[Pesta 2012 Methods Mol Biol]] | ||
* [[ | * [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol]] | ||
* [[ | * [[Sjoevall 2010 CritCare]] | ||
Revision as of 02:10, 10 March 2015
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
SUIT protocols
SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
