Succinate

high-resolution terminology - matching measurements at high-resolution

Succinate

Description

Succinate^2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2.

Abbreviation: S

Reference: MiPNet11.09, MiPNet12.11

MitoPedia topics: Substrate and metabolite 

Labels:

Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.  Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Application in HRR

S: Succinate (Succinate disodium salt, hexahydrate, C₄H₄0₄Na₂); Sigma S 2378, 100 g, store at R.T.): FW = 270.1

Preparation of 1 M stock solution (dissolved in H₂O):

1) weigh 1.3505 g of Succinate and dissolve in 3 ml H₂O

2) check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment)

3) transfer to 5 mL volumetric glass flask and adjust the final volume to 5 ml

4) divide into 0.2 ml portions

5) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 20 µl using a 50 µl syringe
• Final conc. in 2 ml O2k-chamber: 10 mM