Difference between revisions of "Succinate"
From Bioblast
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::4) divide into 0.2 ml portions | ::4) divide into 0.2 ml portions | ||
::5) store frozen at -20 °C | ::5) store frozen at -20 °C | ||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | ||
Revision as of 12:11, 16 May 2012
- high-resolution terminology - matching measurements at high-resolution
Succinate
Description
Succinate2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2.
Abbreviation: S
Reference: MiPNet11.09, MiPNet12.11
MitoPedia topics:
Substrate and metabolite
Application in HRR
S: Succinate (Succinate disodium salt, hexahydrate, C4H404Na2 * (H2O)6; Sigma S 2378, 100 g, store at R.T.; FW = 270.1
Preparation of 1 M stock solution (dissolved in H2O):
- 1) weigh 1.3505 g of Succinate and dissolve in 3 ml H2O
- 2) check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment)
- 3) transfer to 5 mL volumetric glass flask and adjust the final volume to 5 ml
- 4) divide into 0.2 ml portions
- 5) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 20 µl using a 50 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 mM
