Difference between revisions of "Succinate"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''S: Succinate''' (Succinate disodium salt, hexahydrate, C<sub>4</sub>H<sub>4</sub>0<sub>4</sub>Na<sub>2</sub> * (H<sub>2</sub>O)<sub>6</sub>; Sigma S 2378, 100 g, store at R.T.; FW = 270.1 | '''S: Succinate''' (Succinate disodium salt, hexahydrate, C<sub>4</sub>H<sub>4</sub>0<sub>4</sub>Na<sub>2</sub> * (H<sub>2</sub>O)<sub>6</sub>); Sigma S 2378, 100 g, store at R.T.; FW = 270.1 | ||
'''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
Revision as of 12:14, 16 May 2012
- high-resolution terminology - matching measurements at high-resolution
Succinate
Description
Succinate2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2.
Abbreviation: S
Reference: MiPNet11.09, MiPNet12.11
MitoPedia topics:
Substrate and metabolite
Application in HRR
S: Succinate (Succinate disodium salt, hexahydrate, C4H404Na2 * (H2O)6); Sigma S 2378, 100 g, store at R.T.; FW = 270.1
Preparation of 1 M stock solution (dissolved in H2O):
- 1) weigh 1.3505 g of Succinate and dissolve in 3 ml H2O
- 2) check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment)
- 3) transfer to 5 mL volumetric glass flask and adjust the final volume to 5 ml
- 4) divide into 0.2 ml portions
- 5) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 20 µl using a 50 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 mM
