Difference between revisions of "Succinate"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=S | |abbr=S | ||
|description='''Succinate<sup>2-</sup>''' is formed in the [[TCA cycle]], and is a substrate of [[CII]], reacting to fumarate and feeding electrons into the [[Q-junction]] | |description='''Succinate<sup>2-</sup>''' is formed in the [[TCA cycle]], and is a substrate of [[CII]], reacting to fumarate and feeding electrons into the [[Q-junction]]. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. | ||
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet12.11]] | |info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet12.11]] | ||
|type=Respiration | |type=Respiration | ||
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'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | ||
::*Titration volume: 20 µl using a 50 µl syringe | ::* In the absence of CI-linked substrates, add rotenone before addition of succinate, to avoid accumulation of oxaloacetate with subsequent Inhibition of succiante Dehydrogenase. | ||
::*Final | ::* Titration volume: 20 µl using a 50 µl syringe (2 ml O2k-chamber) | ||
::* Final concentration: 10 mM | |||
Revision as of 20:31, 1 June 2013
- high-resolution terminology - matching measurements at high-resolution
Succinate
Description
Succinate2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction.
Abbreviation: S
Reference: Gnaiger 2012 MitoPathways, MiPNet12.11
MitoPedia topics: Substrate and metabolite
Application in HRR
S: Succinate (Succinate disodium salt, hexahydrate, C4H404Na2 * (H2O)6); Sigma S 2378, 100 g, store at R.T.; FW = 270.1
Preparation of 1 M stock solution (dissolved in H2O):
- 1) weigh 1.3505 g of Succinate and dissolve in 3 ml H2O
- 2) check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment)
- 3) transfer to 5 mL volumetric glass flask and adjust the final volume to 5 ml
- 4) divide into 0.2 ml portions
- 5) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- In the absence of CI-linked substrates, add rotenone before addition of succinate, to avoid accumulation of oxaloacetate with subsequent Inhibition of succiante Dehydrogenase.
- Titration volume: 20 µl using a 50 µl syringe (2 ml O2k-chamber)
- Final concentration: 10 mM
