Difference between revisions of "Superoxide dismutase"
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::::# Store frozen at -20 °C in the desiccator. | ::::# Store frozen at -20 °C in the desiccator. | ||
::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[ | ::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[Amplex_UltraRed|Measurement of H<sub>2</sub>O<sub>2</sub> with Amplex UltraRed]]. | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Enzyme | |mitopedia topic=Enzyme | ||
}} | }} |
Revision as of 16:53, 16 September 2020
Description
Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O2•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H2O2.
Abbreviation: SOD
Reference: Fridovich_1997_J Biol Chem
Application in HRR
- SOD: Superoxide dismutase from bovine liver, Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol
- Preparation of 5000 U/mL stock solution (dissolved in H2O)
- Weigh 2 mg of superoxide dismutase.
- Dissolve in 1134 µL H2O.
- Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
- Store frozen at -20 °C in the desiccator.
- Add SOD for conversion of O2•- and Measurement of H2O2 with Amplex UltraRed.
MitoPedia topics: Enzyme