Difference between revisions of "Superoxide dismutase"
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|info=[[Fridovich_1997_J Biol Chem]] | |info=[[Fridovich_1997_J Biol Chem]] | ||
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[[File:O2-.jpg|right|60px|Superoxide anion]] | |||
== Application in [[HRR]] == | |||
::: '''SOD: Superoxide dismutase from bovine liver''', Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol | |||
:::: '''Preparation of 5000 U/mL stock solution''' (dissolved in H<sub>2</sub>O) | |||
:::: Please check the concentration of the batch to calculate the volume needed to obtain a stock solution of 5000 U/mL using 2 mg of superoxide dismutase. | |||
::::# Weigh 2 mg of superoxide dismutase. | |||
::::# Calculate the volume needed to obtain a stock solution of 5000 U/mL (e.g. 1134 µL H<sub>2</sub>O for a batch with a concentration of 2835 U/mg). | |||
::::# Dissolve. | |||
::::# Divide into 30 µL portions into 0.2 mL Eppendorf tubes. | |||
::::# Store frozen at -20 °C in the desiccator. | |||
::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[Amplex_UltraRed|Measurement of H<sub>2</sub>O<sub>2</sub> with Amplex UltraRed]]. | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Enzyme | |mitopedia topic=Enzyme | ||
}} | }} | ||
Latest revision as of 14:20, 7 February 2022
- high-resolution terminology - matching measurements at high-resolution
Superoxide dismutase
Description
Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O2•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H2O2.
Abbreviation: SOD
Reference: Fridovich_1997_J Biol Chem
Application in HRR
- SOD: Superoxide dismutase from bovine liver, Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol
- Preparation of 5000 U/mL stock solution (dissolved in H2O)
- Please check the concentration of the batch to calculate the volume needed to obtain a stock solution of 5000 U/mL using 2 mg of superoxide dismutase.
- Weigh 2 mg of superoxide dismutase.
- Calculate the volume needed to obtain a stock solution of 5000 U/mL (e.g. 1134 µL H2O for a batch with a concentration of 2835 U/mg).
- Dissolve.
- Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
- Store frozen at -20 °C in the desiccator.
- Add SOD for conversion of O2•- and Measurement of H2O2 with Amplex UltraRed.
MitoPedia topics: Enzyme
