Superoxide dismutase: Difference between revisions
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== Application in [[HRFR]] == | == Application in [[HRFR]] == | ||
::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex ultrared]]. | ::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex ultrared]]. | ||
::::* '''SOD: Superoxide dismutase from bovine liver''', Sigma | ::::* '''SOD: Superoxide dismutase from bovine liver''', Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol | ||
::: '''Preparation of 5000 U/ml stock solution''' (dissolved in H<sub>2</sub>O) | ::: '''Preparation of 5000 U/ml stock solution''' (dissolved in H<sub>2</sub>O) | ||
Revision as of 10:59, 15 January 2018
- high-resolution terminology - matching measurements at high-resolution
Superoxide dismutase
Description
Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O2•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H2O2.
Abbreviation: SOD
Reference: Fridovich_1997_J Biol Chem
MitoPedia topics:
Enzyme
Application in HRFR
- Add SOD for conversion of O2•- and measurement of H2O2 with Amplex ultrared.
- SOD: Superoxide dismutase from bovine liver, Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol
- Preparation of 5000 U/ml stock solution (dissolved in H2O)
- Weigh 2 mg of superoxide dismutase.
- Dissolve in 1134 µl H2O.
- Divide into 30 µl portions into 0.2 ml Eppendorf tubes.
- Store frozen at -20 °C in the desiccator.
