TMRM: Difference between revisions
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== Signal and output == | == Signal and output == | ||
# Signal: The [[O2k-Fluo LED2-Module]] is operated through the Amperometric (Amp)-Channel of the O2k, with electric current (Amp) as the primary signal. | # Signal: The [[O2k-Fluo LED2-Module]] is operated through the Amperometric (Amp)-Channel of the O2k, with electric current (Amp) as the primary signal. | ||
# Output: The focus of the output with TMRM is on '''Type C''': Force | # Output: The focus of the output with TMRM is on '''[[Signal and output| Type C]]''': Force, mt-membrane potential | ||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
The TMRM method can be used with the [[O2k-Fluo LED2-Module]]. For examples of the use of TMRM with mouse liver mitochondria, rat liver tissue homogenate, and permeabilized HEK 293T cells see [[Talk:TMRM| the discussion page of this entry]] | The TMRM method can be used with the [[O2k-Fluo LED2-Module]]. For examples of the use of TMRM with mouse liver mitochondria, rat liver tissue homogenate, and permeabilized HEK 293T cells see [[Talk:TMRM| the discussion page of this entry]] | ||
Revision as of 11:47, 28 July 2015
- high-resolution terminology - matching measurements at high-resolution
TMRM
Description
TMRM ( tetramethylrhodamine methyl ester) is an extrinsic fluorophore and can be used as a probe to determine changes in mitochondrial membrane potential. TMRM is a lipophilic cations that is accumulated by mitochondria in proportion to ฮฯmt. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when the dye is accumulated by mitochondria.
Abbreviation: TMRM
Reference: Ehrenberg 1988 Biophys J
MitoPedia methods:
Fluorometry
Signal and output
- Signal: The O2k-Fluo LED2-Module is operated through the Amperometric (Amp)-Channel of the O2k, with electric current (Amp) as the primary signal.
- Output: The focus of the output with TMRM is on Type C: Force, mt-membrane potential
The TMRM method can be used with the O2k-Fluo LED2-Module. For examples of the use of TMRM with mouse liver mitochondria, rat liver tissue homogenate, and permeabilized HEK 293T cells see the discussion page of this entry
