Difference between revisions of "Talk:Glycerophosphate"
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== Popular Bioblast page == | == Popular Bioblast page == | ||
::: [[Glycerophosphate]] has been accessed more than | ::: [[Glycerophosphate]] has been accessed more than | ||
::::* 15,000 times (2019-07-22) | |||
::::* 10,000 times (2016-07-14) | ::::* 10,000 times (2016-07-14) | ||
::::* 5,000 times (2014-11-12) | ::::* 5,000 times (2014-11-12) | ||
== Former Instructions == | |||
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended. | |||
:::: '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | |||
::::# Weigh 324 mg of Gp and dissolve in 1 mL H2O. | |||
::::# Divide into 0.2 mL portions. | |||
::::# Store frozen at -20 °C. | |||
== pH == | == pH == | ||
pH has been check in Glycerophospate (Gp) stock solution (Sigma G7886), showing a high pH (~9). However, the high pH in the Gp stock does not affect the pH in the chamber when adding 20 µl in MiR05 medium. We measured the pH with the O2k-FluoRespirometer. We observed no significant changes (no more than 0.1, see picture below) in the pH after adding 20 µl of Gp to the MiR05-Kit medium. - [[Passrugger M]] 2017-08-31 | |||
:::: [[File:PH Gp.png| | :::: [[File:PH Gp.png|500px|pH measurement of Gp in MiR05 Kit]] | ||
== Glycerophosphate out of stock == | == Glycerophosphate out of stock == | ||
Latest revision as of 13:23, 8 March 2021
Popular Bioblast page
- Glycerophosphate has been accessed more than
- 15,000 times (2019-07-22)
- 10,000 times (2016-07-14)
- 5,000 times (2014-11-12)
- Glycerophosphate has been accessed more than
Former Instructions
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
- Preparation of 1 M stock solution (dissolved in H2O):
- Weigh 324 mg of Gp and dissolve in 1 mL H2O.
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- Preparation of 1 M stock solution (dissolved in H2O):
pH
pH has been check in Glycerophospate (Gp) stock solution (Sigma G7886), showing a high pH (~9). However, the high pH in the Gp stock does not affect the pH in the chamber when adding 20 µl in MiR05 medium. We measured the pH with the O2k-FluoRespirometer. We observed no significant changes (no more than 0.1, see picture below) in the pH after adding 20 µl of Gp to the MiR05-Kit medium. - Passrugger M 2017-08-31
Glycerophosphate out of stock
Sigma G-2138
- Sigma does not sell DL-alpha-glycerophosphate disodium salt hexahydrate (FW = 324.1; Sigma G-2138, CAS# 1555-56-2 (anhydrous)) any more (personal communication with Dr. Zdenek Drahota, Dept. of Bioenergetics, Institute of Physiology, Academy of Sciences of the Czech Rep.)!
- In Dr. Drahota's lab a 1 M stock solution is used and the final concentration in the O2k-Chamber is 10 mM.
Alternatives
- Racemic disodium salt hexahydrate: CAS#: 3325-00-6, "identical" (not considering purity) to the discontinued Sigma product mentioned above, but actual availability would have to be checked with each individual supplier
- CHEMOS: 151924
- BOC Sciences: 3325-00-6 (sic!, they use CAS numbers as their product numbers, which actually makes a lot of sense).
- Advance Scientific & Chemical: G2218.
- Racemic magnesium salt: AppliChem: A4558,1000
- Enantiopure lithium salt: Sigma: 94124
- Enatiopure bis(cyclohexylammonium) salt: Sigma: G7886
- Racemic disodium salt hexahydrate: CAS#: 3325-00-6, "identical" (not considering purity) to the discontinued Sigma product mentioned above, but actual availability would have to be checked with each individual supplier
- Based on a web search the substance CAS 57-03-4 seems to refer to the free acid, probable in as racemic mixture. However, the confusion about Alpha Glycerol Phosphate is so widespread that even ß-glycerolphosphate (a definitively different compound !!) can be found with the same CAS number on the web...
- The compound from Sigma (that was used previously and is no longer available) was the sodium salt of the racemat (CAS# 1555-56-2 (anhydrous), CAS#: 3325-00-6 (hexahydrate, maybe more CAS numbers) see: Glycerophosphate
- If my guess in respect to CAS 57-03-4 is correct (free acid, racemic) this would be a reasonable substitution for the racemic sodium form, only with a bit more work to prepare the stock solution. As you can see from the link there are some companies offering the racemic sodium salt, however we have not yet checked if the substance is REALLY available and we have not done any application tests. - Fasching Mario 2014-07-09
