Difference between revisions of "Talk:Raw signal of the oxygen sensor"
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::::* at Gain 4 : ca. 8 - 10 V (do not use at air saturation, especially if your lab is situated at or near sea level) | ::::* at Gain 4 : ca. 8 - 10 V (do not use at air saturation, especially if your lab is situated at or near sea level) | ||
::::* at Gain 2: ca. 4 - 5 V | ::::* at Gain 2: ca. 4 - 5 V | ||
::::* at Gain 1: ca. | ::::* at Gain 1: ca. 1 - 3 V | ||
:::: The raw signal observed during calibration at air saturation depends on experimental temperature, barometric pressure (lower values are obtained above sea level), and the sensitivity of the individual POS. The raw signal is converted to oxygen concentration [µM or nmol/ml] or partial oxygen pressure [kPa] by calibration of the POS [[MiPNet12.08]]. Each gain requires a separate calibration of the oxygen signal. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero oxygen calibration. | :::: The raw signal observed during calibration at air saturation depends on experimental temperature, barometric pressure (lower values are obtained above sea level), and the sensitivity of the individual POS. The raw signal is converted to oxygen concentration [µM or nmol/ml] or partial oxygen pressure [kPa] by calibration of the POS [[MiPNet12.08]]. Each gain requires a separate calibration of the oxygen signal. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero oxygen calibration. | ||
{{#set:Technical service=O2 sensor|Technical service=O2 signal}} | {{#set:Technical service=O2 sensor|Technical service=O2 signal}} | ||
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Talk:Raw signal of the oxygen sensor |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- The raw signal of the polarographic oxygen sensor (POS) depends on the gain setting (default is Gain 1 in O2k-Series G, gain 2 in previous Series). The maximum signal is 10 volt. Above 9.99 V, the amplifier is in the saturation range, the apparent signal will remain constant at this value, and the slope will appear to be zero. The raw signal can be modified by choosing an appropriate gain setting.
- Typical recorded voltages at air saturation are
- at Gain 8: offscale ( “9.99V”)
- at Gain 4 : ca. 8 - 10 V (do not use at air saturation, especially if your lab is situated at or near sea level)
- at Gain 2: ca. 4 - 5 V
- at Gain 1: ca. 1 - 3 V
- Typical recorded voltages at air saturation are
- The raw signal observed during calibration at air saturation depends on experimental temperature, barometric pressure (lower values are obtained above sea level), and the sensitivity of the individual POS. The raw signal is converted to oxygen concentration [µM or nmol/ml] or partial oxygen pressure [kPa] by calibration of the POS MiPNet12.08. Each gain requires a separate calibration of the oxygen signal. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero oxygen calibration.
