Talk:SUIT-006 MgG mt D055: Difference between revisions
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Revision as of 10:42, 31 August 2020
Chemicals and syringes needed
- Magnesium Green (MgG)
- MgCl2 (Mg)
- Pyruvate (P)
- Malate (M)
- ADP (D)
- Carboxyatractyloside (Cat), can be substituted for Oligomycin
- Uncoupler: in this DLP, SF6847 was used, can be substituted for other uncoupler (U)
- Antimycin A (Ama)
| Chemical and link | Abbreviation | Comments |
|---|---|---|
| Magnesium Green | MgG | |
| MgCl2 | Mg |
| Chemical and link | Abbreviation | Comments |
|---|---|---|
| Pyruvate | P | Can be substituted by other combination of substrates |
| Malate | M | Can be substituted by other combination of substrates |
| ADP | D | For this protocol, the ADP must be prepared without Mg2+ salts. |
| Carboxyatractyloside | Cat | Can be substituted for Oligomycin |
| Uncoupler: in this DLP, SF6847 was used | U | Can be substituted for other uncoupler |
| Antimycin A | Ama | Can be substituted for other CIII or CIV inhibitor |
