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Difference between revisions of "Talk:SUIT-006 MgG mt D055"

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(Created page with "== Chemicals and syringes needed == :::* Magnesium Green (MgG) :::* Magnesium_Green#Preparation_of_other_solutions|MgCl<sub>...")
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== Chemicals and syringes neededย  ==
== Chemicals and syringes neededย  ==
:::* [[Magnesium_Green#Preparation_of_MgG_solution|Magnesium Green]] (MgG)
:::* [[Magnesium_Green#Preparation_of_other_solutions|MgCl<sub>2</sub>]] (Mg)
:::* [[Pyruvate#Application_in_HRR|Pyruvate]] (P)
:::* [[Malate#Application_in_HRR|Malate]] (M)
:::* [[Magnesium_Green#Preparation_of_other_solutions|ADP]] (D)
:::* [[Carboxyatractyloside#Application_in_HRR|Carboxyatractyloside]] (Cat), can be substituted for [[Oligomycin#Application_in_HRR|Oligomycin]]
:::* Uncoupler: in this DLP, [[SF6847#Application_in_HRR|SF6847]] was used, can be substituted for other [[MitoPedia:_Uncouplers|uncoupler]] (U)
:::* [[Antimycin_A#Preparation_of_other_solutions|Antimycin A]] (Ama)


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Revision as of 10:46, 31 August 2020

Chemicals and syringes needed

Chemical and link Abbreviation Comments
Magnesium Green MgG
MgCl2 Mg
Chemical and link Abbreviation Comments
Pyruvate P Can be substituted by other combination of substrates
Malate M Can be substituted by other combination of substrates
ADP D For this protocol, the ADP must be prepared without Mg2+ salts.
Carboxyatractyloside Cat Can be substituted for Oligomycin
Uncoupler: in this DLP, SF6847 was used U Can be substituted for other uncoupler
Antimycin A Ama Can be substituted for other CIII or CIV inhibitor