Talk:Zhang Feiyuan
| Description | DatLab single licence incl. MitoFit Proficiency Test, containing our most user-friendly software DatLab 7.3 and MitoFit Proficiency Test for High-Resolution FluoRespirometry as well as the Oroboros Premium Customer Support. Upon successful completion of the test, a MitoFit Proficiency Test certificate from Oroboros Instruments acknowledging your personal proficiency in the own laboratory environment will be conferred. As standard components of the package, MiR05-Kit, cryopreserved HEK 293T cells and chemicals for the Proficiency Test are included. A test for up to three participants from the same research group is included in the price. |
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| Product ID | 70050-01 |
| Type | O2k |
| Link |  |
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MitoFit Proficiency Test
Oroboros Instruments presents the MitoFit Proficiency Test, the new standard in quality control in mitochondrial physiology. The introductory offer contains the following unique components:
- DatLab 7.3 for High-Resolution FluoRespirometry. Our most user-friendly software than ever.
- You receive the fully functional DatLab 7.3 software with 10 SUIT DatLab-Protocols (DLPs), Excel sheets for data analysis, necessary Instrumental DLPs for air and zero oxygen calibration and for determination of the instrumental background oxygen flux.
- MitoFit Proficiency Test for High-Resolution FluoRespirometry
- A three-level proficiency test, with
- (1)Instrumental proficiency: instrumental background and air calibration to ensure optimum instrumental performance
- (2)SUIT protocols with permeabilized cells
- (3)Data analysis by the user
- The Proficiency Test is based on the user-friendly DatLab-Protocols. The test evaluation is conducted by mitochondrial physiology experts at Oroboros Instruments.
- Exclusive Oroboros Premium Customer Support included in the introductory package
- You receive detailed feedback on the quality of measurement and analysis within the Proficiency Test.
- Technical and scientific supports are provided by mitochondrial physiology experts at Oroboros Instruments in case of issues during the test.
- Certification upon successful test
A MitoFit Proficiency Test certificate from Oroboros Instruments acknowledging your personal proficiency in the own laboratory environment will be conferred.
- Consumables: MiR05-Kit, cryopreserved HEK 293T cells and chemicals for the Proficiency Test
- You receive the pre-prepared respiration medium MiR05-Kit; cryopreserved cells, ready to be measured in the O2k and certified chemicals to minimize inter-laboratory differences and increase reproducibility.
- *1 vial MiR05-Kit per research group and 2 vials of cryopreserved HEK 293T cells per participant are included.
- Store the second vial of cells at -80 °C until our feedback on your performance. It can be used for a second test if the first one is not successful.
With the financial support by MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur TirolSpecial the introductory offer is valid until 2018-10-30. ?
Not for Product Catalogue
Guidelines for the MitoFit Proficiency Test
Registration sheet: #### (PDF zum Herunterladen)?
Please indicate if you are a beginner or an advanced O2k user (guidelines for self-assessment: ####)?
Equipment:
- Oroboros O2k, Series A-H (2004 – 2018)
- DatLab 7.3 (provided)
- PC / laptop, Windows XP+
- Titration Set with Hamilton syringes:
- HEK 293T cells (provided)
- Chemicals
Experiments are to be performed individually (one O2k instrument per participant), without any further help. Manuals and SOPs can be used.
Material:
- HEK cells, titrated with the “Sample” Hamilton syringe
- Respiration medium: MiR05-Kit (provided)?
- SUIT 1 and 2 pce (D## and D##)?
Settings:
- Set slope smoothing to 20 data points at the beginning the measurement
- Use DL-Protocol for air calibration.
- Use low O2 warning function and set it to 30 µM.
- Add MiR05-Kit lot number, preparation date, cell lot and preparation date in the Experiment/Sample/Comments field
- Theoretical cell count: 1 million cells/mL
- Routine respiration: wait until steady-state, but max 15 min
- RP1: ADP titration (2*10 uL)
- Reoxygenate before uncoupler titration
Data evaluation
- Make a copy of your aircal and RP1-RP2 files into the folder "PT Evaluated".
- Make a copy of the two Excel files \SRV1\Lab\3C_Projects_Data\2017-All Data\2017_Proficiency_test_DSL (Preprint) for evaluation in your personal "PT Evaluated" folder.
- Check the measurement files if all settings are correct, events are set correctly, etc.
- Set marks and copy data to clipboard.
- Use the Excel files for data evaluation. Do not forget to set the proper Sample number and Subsample number you used on tabs "Protocol", "Systematic" and "FCR".
Upon execution of the measurement and data analysis by the participants, DatLab 7.3 (DLD) files with the accompanying MS Excel templates for analysis (provided with the SUIT package) should be submitted to the following website: #####
The measurements are to be anonymously analyzed by the mitochondrial physiology experts at Oroboros Instruments.
