Template:SUIT text D030: Difference between revisions
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The SUIT-007 O2 pce D030 is focused on the [[Glutamate anaplerotic pathway control state|glutamate anaplerotic pathway]]. If [[Glutamate dehydrogenase|glutamate dehydrogenase]] is present in the sample when glutamate is added alone, it will be converted to α-ketoglutarate in an [[Anaplerosis|anaplerotic reaction]], supporting respiration. After malate is added, it is possible to analyse the [[NADH Electron transfer-pathway state]] with glutamate and malate as substrates in the [[OXPHOS]] and [[ET]] [[Coupling control state|coupling control states]]. | The SUIT-007 O2 pce D030 is focused on the [[Glutamate anaplerotic pathway control state|glutamate anaplerotic pathway]]. If [[Glutamate dehydrogenase|glutamate dehydrogenase]] is present in the sample when glutamate is added alone, it will be converted to α-ketoglutarate in an [[Anaplerosis|anaplerotic reaction]], supporting respiration. After malate is added, it is possible to analyse the [[NADH Electron transfer-pathway state]] with glutamate and malate as substrates in the [[OXPHOS]] and [[ET]] [[Coupling control state|coupling control states]]. | ||
In this protocol, non-permeabilized cells are added in the chamber, and [[ROUTINE]] respiration is measured. The cells are further permeabilized with [[digitonin]] inside the O2k chamber. | |||
Revision as of 16:12, 19 July 2019
The SUIT-007 O2 pce D030 is focused on the glutamate anaplerotic pathway. If glutamate dehydrogenase is present in the sample when glutamate is added alone, it will be converted to α-ketoglutarate in an anaplerotic reaction, supporting respiration. After malate is added, it is possible to analyse the NADH Electron transfer-pathway state with glutamate and malate as substrates in the OXPHOS and ET coupling control states. In this protocol, non-permeabilized cells are added in the chamber, and ROUTINE respiration is measured. The cells are further permeabilized with digitonin inside the O2k chamber.
