Difference between revisions of "Thomas 2011 Mitochondrion"
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|injuries=RONS; Oxidative Stress | |injuries=RONS; Oxidative Stress | ||
|organism=Human, Mouse | |organism=Human, Mouse | ||
|tissues= | |tissues=Heart, Skeletal muscle, Nervous system | ||
|model cell lines=Fibroblast | |model cell lines=Fibroblast | ||
|preparations=Homogenate, Isolated Mitochondria | |preparations=Homogenate, Isolated Mitochondria | ||
|topics=ATP; ADP; AMP; PCr | |topics=ATP; ADP; AMP; PCr | ||
}} | }} | ||
Revision as of 12:12, 27 February 2013
| Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP Jr (2011) Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11: 108-118. |
Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP (2011) Mitochondrion
Abstract: Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP+)-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, Complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis. • Keywords: recombinant TFAM, mitochondrial biogenesis, ATP synthesis, MPTP, sepsis
• O2k-Network Lab: US_VA Richmond_Bennett JP, US VA Richmond Virginia Commonwealth Univ
Labels:
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Human, Mouse Tissue;cell: Heart, Skeletal muscle, Nervous system Preparation: Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
