Difference between revisions of "Torelli 2015 Free Radic Biol Med"
Beno Marija (talk | contribs) |
Beno Marija (talk | contribs) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 18: | Line 18: | ||
induction of antioxidant defenses and stress resistance. | induction of antioxidant defenses and stress resistance. | ||
|keywords=Retrograde signaling, Hormesis, Mitochondria, H2O2, Safranin, Amplex Red | |keywords=Retrograde signaling, Hormesis, Mitochondria, H2O2, Safranin, Amplex Red | ||
|mipnetlab=BR Sao Paulo Kowaltowski AJ | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
| Line 23: | Line 24: | ||
|organism=Saccharomyces cerevisiae | |organism=Saccharomyces cerevisiae | ||
|preparations=Intact cells, Isolated mitochondria | |preparations=Intact cells, Isolated mitochondria | ||
|couplingstates=LEAK, ROUTINE, OXPHOS, | |couplingstates=LEAK, ROUTINE, OXPHOS, ET | ||
|pathways=CIV, ROX | |pathways=CIV, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 17:14, 27 March 2018
| Torelli NQ, Ferreira-Júnior JR, Kowaltowski AJ, da Cunha FM (2015) RTG1- and RTG2-dependent retrograde signaling controls mitochondrial activity and stress resistance in Saccharomyces cerevisiae. Free Radic Biol Med 81C:30-7. |
Torelli NQ, Ferreira-Junior JR, Kowaltowski AJ, da Cunha FM (2015) Free Radic Biol Med
Abstract: Mitochondrial retrograde signaling is a communication pathway between the mitochondrion and the nucleus which regulates the expression of a subset of nuclear genes that codify mitochondrial proteins, mediating cell response to mitochondrial dysfunction. In Saccharomyces cerevisiae, the pathway depends on Rtg1p and Rtg3p, which together form the transcription factor that regulates gene expression, and Rtg2p, an activator of the pathway. Here, we provide novel studies aimed at assessing the functional impact of the lack of RTG-dependent signaling on mitochondrial activity. We show that mutants defective in RTG dependent retrograde signaling present higher oxygen consumption and reduced hydrogen peroxide release in the stationary phase when compared to wild type cells. Interestingly, RTG mutants are less able to decompose hydrogen peroxide as well as maintain viability when challenged with hydrogen peroxide. Overall, our results indicate that RTG signaling is involved in the hormetic induction of antioxidant defenses and stress resistance. • Keywords: Retrograde signaling, Hormesis, Mitochondria, H2O2, Safranin, Amplex Red
• O2k-Network Lab: BR Sao Paulo Kowaltowski AJ
Labels: MiParea: Respiration, mt-Membrane, Genetic knockout;overexpression
Organism: Saccharomyces cerevisiae
Preparation: Intact cells, Isolated mitochondria
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: CIV, ROX
HRR: Oxygraph-2k
