User talk:Spitzer Gudrun

Asc gutest 1

As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.

Caution: Light sensitive (store solution in a dark glass vial).

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Preparation of 800 mM stock solution (dissolved in H₂O) for use in 2-mL O2k-chamber:

1. Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H₂O
2. To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL^-1 solution of pH 2).
3. Divide into 0.2 mL portions.
4. Store frozen at -20 °C.

Preparation of 1000 mM stock solution (dissolved in H₂O) for use in 0.5-mL O2k-chamber:

1. Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H₂O
2. To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL^-1 solution of pH 2).
3. Divide into 0.2 mL portions.
4. Store frozen at -20 °C.

»O2k manual titrations: MiPNet09.12

• Titration volume (2-mL O2k-chamber): 5 µL of 800mM stock solution using a 25 µL Hamilton syringe.
• Titration volume (0.5-mL O2k-chamber): 1 µL of 1000mM stock solution using a 10 µL Hamilton syringe
• Final concentration: 2 mM.

Asc gutest 2

As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.

Caution: Light sensitive (store solution in a dark glass vial).

Preparation of 800 mM stock solution (dissolved in H₂O) for 2-mL O2k-chamber:

1. Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H₂O
2. To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL^-1 solution of pH 2).
3. Divide into 0.2 mL portions.
4. Store frozen at -20 °C.

»O2k manual titrations: MiPNet09.12

• Titration volume (2-mL O2k-chamber): 5 µL of 800 mM stock solution using a 25 µL Hamilton syringe.
• Final concentration: 2 mM.

Preparation of 1000 mM stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

1. Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H₂O
2. To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL^-1 solution of pH 2).
3. Divide into 0.2 mL portions.
4. Store frozen at -20 °C.

»O2k manual titrations: MiPNet09.12

• Titration volume (0.5-mL O2k-chamber): 1 µL of 1000 mM stock solution using a 10 µL Hamilton syringe.
• Final concentration: 2 mM.

Version 3

As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.

Caution: Light sensitive (store solution in a dark glass vial).

Preparation of 800 mM stock solution (dissolved in H₂O) for 2-mL O2k-chamber:

1. Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H₂O
2. To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL^-1 solution of pH 2).
3. Divide into 0.2 mL portions.
4. Store frozen at -20 °C.

»O2k manual titrations: MiPNet09.12

• Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
• Final concentration: 2 mM.

Preparation of 1000 mM stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

1. Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H₂O
2. To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL^-1 solution of pH 2).
3. Divide into 0.2 mL portions.
4. Store frozen at -20 °C.

»O2k manual titrations: MiPNet09.12

• Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 2 mM.