Difference between revisions of "Valencia 2023 MiP2023"
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{{Abstract | {{Abstract | ||
|title=T-cell mitochondria exhibit a functional decline with obesity that is aggravated by weight loss. | |title=[[Image:MiPsocietyLOGO.JPG|left|100px|Mitochondrial Physiology Society|MiPsociety]]T-cell mitochondria exhibit a functional decline with obesity that is aggravated by weight loss. | ||
|info=[[MiP2023 Obergurgl AT]] | |info=[[MiP2023 Obergurgl AT]] | ||
|authors=Valencia Ana P | |authors=Valencia Ana P | ||
|year=2023 | |year=2023 | ||
|event=MiP2023 Obergurgl AT | |event=MiP2023 Obergurgl AT | ||
|abstract=Authors: Valencia Ana P, Melhorn Susan J, Schur Ellen A, Marcinek David J<br> | |abstract='''Authors:''' [[Valencia Ana P]], [[Melhorn Susan J]], [[Schur Ellen A]], [[Marcinek David J]]<br><br> | ||
Introduction: Weight loss (WL) promotes counterregulatory mechanisms that may involve mitochondrial (MITO) function to limit cardiometabolic benefit. This study compared T-cell MITO function in states of obesity (OB), active weight loss (OB-WL), weight loss plateau (OB-PL), regain (OB-RG), and healthy weight (HWC).<br> | '''Introduction:''' Weight loss (WL) promotes counterregulatory mechanisms that may involve mitochondrial (MITO) function to limit cardiometabolic benefit. This study compared T-cell MITO function in states of obesity (OB), active weight loss (OB-WL), weight loss plateau (OB-PL), regain (OB-RG), and healthy weight (HWC).<br> | ||
Methods: Participants with obesity (61.5%female, 39.5±10.8 yr, BMI 36.7±6.4) underwent a 24-week WL intervention and transmitted their daily weight for 18 months. T-cells (CD3+) were isolated from blood samples obtained at baseline, 6-month, or 12-months. We measured MITO respiratory capacity (MITO-RC) (XFe Analyzer) and sensitivity of membrane depolarization with ADP (IC50) (O2K Fluorespirometer).<br> | '''Methods:''' Participants with obesity (61.5%female, 39.5±10.8 yr, BMI 36.7±6.4) underwent a 24-week WL intervention and transmitted their daily weight for 18 months. T-cells (CD3+) were isolated from blood samples obtained at baseline, 6-month, or 12-months. We measured MITO respiratory capacity (MITO-RC) (XFe Analyzer) and sensitivity of membrane depolarization with ADP (IC50) (O2K Fluorespirometer).<br> | ||
'''Results:''' Compared to HWC, MITO-RC was lower in OB T-cells (4.1±1.7 vs. 3.3±1.0 pmol O<sub>2</sub>/10<sup>6</sup> cells, p<0.05), and even lower in OB-PL (3.0±0.7, p<0.05) and OB-RG (2.7±0.3, p<0.05). Maximal membrane potential was also lower in the OB group and remained low in all phases of WL. IC50 did not differ in T-cells between HWC and OB but was lower in OB-WL and OB-PL (156±15 vs. 7±1 & 22±5, p<0.05).<br> | |||
Conclusions: T-cell MITO respiratory capacity is reduced in obesity and further aggravated in response to WL, particularly following a plateau. However, WL improved ADP sensitivity, suggesting a potential counterregulatory mechanism to meet energy demand. Findings suggest that the MITO function of T-cells is not restored by WL to resemble HWC and is rather altered in a way that could potentially limit cardiometabolic benefit of WL. | '''Conclusions:''' T-cell MITO respiratory capacity is reduced in obesity and further aggravated in response to WL, particularly following a plateau. However, WL improved ADP sensitivity, suggesting a potential counterregulatory mechanism to meet energy demand. Findings suggest that the MITO function of T-cells is not restored by WL to resemble HWC and is rather altered in a way that could potentially limit cardiometabolic benefit of WL.<br> | ||
|keywords=ADP sensitivity, peripheral mononuclear cells, weight loss plateau | |keywords=ADP sensitivity, peripheral mononuclear cells, weight loss plateau, metabolic adaptation | ||
|mipnetlab=US WA Seattle Valencia A | |mipnetlab=US WA Seattle Valencia A | ||
}} | }} | ||
== Figures == | |||
[[File:Valencia 2023 MiP2023 Figure.png|400px|none|thumb|'''Figure.''' Differences in respiratory capacity and ADP sensitivity in T-cells in response to obesity and different phases of weight loss]] | |||
== Affiliations == | |||
:::: Valencia Ana P<sup>1,2</sup>, Melhorn Susan J<sup>3</sup>, Schur Ellen A<sup>3</sup>, Marcinek David J<sup>1</sup> | |||
::::# Dept of Radiology, Univ of Washington, Seattle, WA, USA | |||
::::# Div of Metabolism, Endocrinology, and Nutrition, Univ of Washington, Seattle, WA, USA | |||
::::# Div of General Internal Medicine, Dept of Medicine, Univ of Washington, Seattle, WA, USA | |||
:::: Corresponding author: apv4@uw.edu | |||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Membrane | |area=Respiration, mt-Membrane | ||
Line 19: | Line 29: | ||
|tissues=Blood cells | |tissues=Blood cells | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|event= | |event=Oral | ||
}} | }} |
Latest revision as of 16:48, 12 July 2023
Valencia 2023 MiP2023
T-cell mitochondria exhibit a functional decline with obesity that is aggravated by weight loss. |
Link: MiP2023 Obergurgl AT
Valencia Ana P (2023)
Event: MiP2023 Obergurgl AT
Authors: Valencia Ana P, Melhorn Susan J, Schur Ellen A, Marcinek David J
Introduction: Weight loss (WL) promotes counterregulatory mechanisms that may involve mitochondrial (MITO) function to limit cardiometabolic benefit. This study compared T-cell MITO function in states of obesity (OB), active weight loss (OB-WL), weight loss plateau (OB-PL), regain (OB-RG), and healthy weight (HWC).
Methods: Participants with obesity (61.5%female, 39.5±10.8 yr, BMI 36.7±6.4) underwent a 24-week WL intervention and transmitted their daily weight for 18 months. T-cells (CD3+) were isolated from blood samples obtained at baseline, 6-month, or 12-months. We measured MITO respiratory capacity (MITO-RC) (XFe Analyzer) and sensitivity of membrane depolarization with ADP (IC50) (O2K Fluorespirometer).
Results: Compared to HWC, MITO-RC was lower in OB T-cells (4.1±1.7 vs. 3.3±1.0 pmol O2/106 cells, p<0.05), and even lower in OB-PL (3.0±0.7, p<0.05) and OB-RG (2.7±0.3, p<0.05). Maximal membrane potential was also lower in the OB group and remained low in all phases of WL. IC50 did not differ in T-cells between HWC and OB but was lower in OB-WL and OB-PL (156±15 vs. 7±1 & 22±5, p<0.05).
Conclusions: T-cell MITO respiratory capacity is reduced in obesity and further aggravated in response to WL, particularly following a plateau. However, WL improved ADP sensitivity, suggesting a potential counterregulatory mechanism to meet energy demand. Findings suggest that the MITO function of T-cells is not restored by WL to resemble HWC and is rather altered in a way that could potentially limit cardiometabolic benefit of WL.
• Keywords: ADP sensitivity, peripheral mononuclear cells, weight loss plateau, metabolic adaptation
• O2k-Network Lab: US WA Seattle Valencia A
Figures
Affiliations
- Valencia Ana P1,2, Melhorn Susan J3, Schur Ellen A3, Marcinek David J1
- Dept of Radiology, Univ of Washington, Seattle, WA, USA
- Div of Metabolism, Endocrinology, and Nutrition, Univ of Washington, Seattle, WA, USA
- Div of General Internal Medicine, Dept of Medicine, Univ of Washington, Seattle, WA, USA
- Corresponding author: apv4@uw.edu
- Valencia Ana P1,2, Melhorn Susan J3, Schur Ellen A3, Marcinek David J1
Labels: MiParea: Respiration, mt-Membrane
Pathology: Obesity
Organism: Human Tissue;cell: Blood cells
HRR: Oxygraph-2k Event: Oral