Difference between revisions of "Van Bergen 2014 Mitochondrion"
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|tissues=Nervous system, Blood cells | |tissues=Nervous system, Blood cells | ||
|model cell lines=Lymphocyte | |model cell lines=Lymphocyte | ||
|preparations=Intact cells, Permeabilized cells, Isolated | |preparations=Intact cells, Permeabilized cells, Isolated mitochondria | ||
|enzymes=Complex I, Complex II; | |enzymes=Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Marker Enzyme | ||
|topics=ATP production, Uncoupler | |topics=ATP production, Uncoupler | ||
|couplingstates=LEAK, ROUTINE, OXPHOS, ETS | |couplingstates=LEAK, ROUTINE, OXPHOS, ETS | ||
|substratestates=CI, CII, CI | |substratestates=CI, CII, CI&II | ||
|instruments=Oxygraph-2k, TIP2k, Protocol | |instruments=Oxygraph-2k, TIP2k, Protocol | ||
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Revision as of 16:17, 10 February 2015
| Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33. |
Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Mitochondrion
Abstract: Mitochondrial oxidative phosphorylation (OXPHOS) dysfunction is implicated in a growing spectrum of diseases, from neurodegeneration to cancer. Where tissues or transformed cells are available, respirometry and enzymology allow a sophisticated analysis of OXPHOS with modest-cost equipment. The isolation of organelle fractions is also invaluable for determining association of proteins of interest. Here we revisit and consolidate methods to measure whole cell mitochondrial ATP synthesis, respiration, isolation of mitochondria from cultured cells and tissues, and OXPHOS enzymology. We also explain common pitfalls, guide optimisation of the methods for new users, and provide full laboratory protocols in Supplementary materials.
• O2k-Network Lab: AU Melbourne Trounce IA
Labels: MiParea: Respiration, Instruments;methods, mtDNA;mt-genetics, mt-Medicine
Organism: Human, Mouse
Tissue;cell: Nervous system, Blood cells
Preparation: Intact cells, Permeabilized cells, Isolated mitochondria
Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Marker Enzyme"Marker Enzyme" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
Regulation: ATP production, Uncoupler
Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, TIP2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Publications
>>Bioblast_alert_2014(02)
